Our team is interested in macrocomplexes of the bacterial enveloppe that energize essential functions in bacteria, such as cell division and transport across the membranes. We use Escherichia coli and the human pathogen Vibrio cholerae as a model organisms to explore the structure and function of two of these conserved molecular motors: the Tol-Pal and the TonB-ExbB-ExbD systèmes.


Tol-Pal, Tol and Ton molecular motor, protein-protein interactions, proton-motive force, cell division, colicins, filamentous phages, host-phage interaction 


Biochemistry, Genetics, Microbiology, Microscopy, Molecular Biology


about 400 hrs/year

at Aix-Marseille University :

Biochemistry, Microbiology, Synthetic Biology, Genetics


S. Buchanan, NIDDK, NIH, Bethesda, MD, USA

T. Mignot, LCB UMR7283, Marseille, France

Our lab is part of the French network
Fur -Dam regulatory interplay at an internal promoter of the enteroaggregative E. coli Type VI secretion sci1 gene cluster. Yannick R Brunet, Christophe S Bernard, E. Cascales. Journal of Bacteriology (2020)

Decoupling filamentous phage uptake and energy of the TolQRA motor in 
Escherichia coli. Samire P, Serrano B, Duche D, Lemarie E, Lloubes R, Houot L. J Bacteriol. 2019 Oct 21.

Cryo-EM structure of the bacterial Ton motor subcomplex ExbB-ExbD provides information on structure and stoichiometry. Celia H, Botos I, Ni X, Fox T, De Val N, Lloubes R, Jiang J, Buchanan SK. Nature Commun Biol. 2019 Oct 4;2:358.
Tol Energy-Driven Localization of Pal and Anchoring to the Peptidoglycan Promote Outer-Membrane Constriction. Petiti M, Serrano B, Faure L, Lloubes R, Mignot T, Duché D. J Mol Biol. 431(17):3275-3288, 2019

Recent publications

Capture d’écran 2020-05-14 à 10.21.42.